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Eur J Endocrinol (2011) 164:58590

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Primers were designed to fit in resulting vector, pJB-HTS upstream of the hexa-histadine tag (5/phos/CCATGGGCAGCAGCCATCATCAT), and downstream (AGCTGGAATTCCTAGTTATTGCTCAGCGGTGGC) (Integrated DNA technologies) of the spacer yielding a fragment containing a phosphorylated 5 end, an initiation codon, a hexa-histadine tag, a thrombin cleavable sequence, a spacer, a termination codon, and an EcoRI site

It is worth mentioning that, GLP-1 inhibits glucagon secretion in -cells during hyperglycemia and normal glycemia states, but does not have this effect at low glycemia levels

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